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ABI Power SYBR™ Green PCR Ma

ster Mix
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  • 询价
  • ABI
  • 美国
  • 4367659
  • 2025年12月21日
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    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 保存条件

      Store at -20°C

    • 保质期

      1年

    • 英文名

      Power SYBR™ Green PCR Master Mix

    • 库存

      大量现货库存

    • 供应商

      朗智(上海)生物科技有限公司

    • 规格

      5ml

    Description

    Get superior nucleic acid quantitation with Applied Biosystems® Power SYBR® Green Master Mix. Power SYBR® Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

    • Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient single-tube format. Power SYBR® Green PCR Master Mix contains all of the components, excluding the template and primers, for superior SYBR® Green reagent-based real-time PCR
    • The optimized formulation contains highly purified AmpliTaq Gold® DNA Polymerase, LD to offer greater sensitivity than classic SYBR® Green PCR master mix
    Power SYBR® Green PCR Master Mix contains a blend of dTTP/dUTP that is compatible with AmpErase® UNG, to minimize carryover contamination
    • Includes a proprietary version of ROX™ dye, an internal passive reference, to normalize non-PCR–related fluorescence fluctuations to minimize well-to-well variability that result from a variety of causes, such as pipetting error and sample evaporation
    Power SYBR® Green reagent-based PCR chemistry easily replaces the existing SYBR® Green PCR Master Mix in Applied Biosystems® protocols using the same setup and thermal cycling conditions

    Alternative product
    Try  PowerUP SYBR Green Master Mix,our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Power SYBR Green PCR Master Mix and added additional capabilities for your gene expression analysis.

    Specifications

    Detection Method:SYBR
    For Use With (Equipment):7300 System,7500 System,7700 System,7900HT Fast System,QuantStudio™ 12k Flex,QuantStudio™ 3,QuantStudio™ 5,QuantStudio™ 6 Flex,QuantStudio™ 7,StepOne™, Standard Mode,StepOnePlus™, Standard Mode,ViiA™ 7 System
    Label or Dye:SYBR® Green
    PCR Method:qPCR
    Passive Reference Dye:ROX (Pre-mixed)
    Polymerase:AmpliTaq Gold DNA Polymerase
    Primer-Probe Compatibility:Unlabeled Oligos
    Product Line:SYBR®
    Product Size:1 x 5 mL
    Purity or Quality Grade:LD (Low DNA)
    Reaction Speed:Standard
    Sample Type (General):DNA
    Sample Type (Specific):DNA (Genomic),cDNA
    Shipping Condition:Wet Ice

    Contents & storage

    The 2X mix contains SYBR® Green 1 Dye, AmpliTaq Gold DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference 1, and optimized buffer components. Contains 1 × 5 mL, sufficient for 200 reactions of 50 µl each.

    Store at -20°C.

    For Research Use Only. Not for use in diagnostic procedures.

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    图标文献和实验
    相关实验
    • SYBR Green Quantitative PCR Protocol

      12μl of master mix for each well, plus some excess1 Rxn: 10μl SYBR Green Mix 52 Rxn: 520μl SYBR 0.4μl Forward Primer (10μM stock) 20.8μl F

    • REAL TIME PCR WITH SYBR GREEN I 建议PROTOCOL

      1、反应体系 25ulCocktail 20 ul: Primer FP 1 ul + Primer RP 1u + 2 * SYBR GREEN I MIX 12.5 + H2O 5.5 ul cDNA 5 ul2、Primer 浓度,需要摸索,从200nM到700nm等,设置不同浓度。3、每个引物浓度,从well 1到12设置4个样本,阳性cDNA 1和2,NTC以及H2O,做triplicate。4、若用ABI7000或7700,所有的PCR条件可设置成一致的,减少麻烦,当然,引物

    • Real time PCR-ABI Prism 7000

        ABI7000 use notes  ,131k,word文档 http://www.wi.mit.edu/CMT/protocols/ABI7000RT_vCMT1.doc   Primer Express ,96页,PDF格式 http://www.wi.mit.edu/CMT/protocols/ABI7000RT_vCMT1.doc   SYBR® Green PCR

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