- ¥1000
- HZbscienc
- 中国/欧洲/美国
- HZ0102
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20摄氏度保存
- 保质期：
1年
- 英文名：
pEGFP-Actin
- 库存：
大量
- 供应商：
上海沪震生物科技有限公司
- 规格：
盒
pEGFP-Actin产品信息
订购信息
| 货号 | 载体名称 | 出品公司 | 载体用途 | 价格 | 到货周期 |
| HZ0102 | pEGFP-Actin | 荧光蛋白报告载体 | 1000 | 现货 |
产品参数
测序引物：T7 Fwd
真核筛选标记：Neomycin
载体描述
pEGFP-Actin encodes a fusion protein consisting of the red-shifted, human codon-optimized variant of green fluorescent protein (EGFP) and the gene encoding human cytoplasmic b-actin. EGFP, a derivative of the GFPmut1 variant, has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) This variant contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr, and also contains more than 190 silent base changes which correspond to human codon-usage preferences. SV40 polyadenylation signals downstream of the EGFP-Actin fusion direct proper processing of the 3' end of the EGFP mRNA. The vector backbone also contains an SV40 origin for replication in any mammalian cell line that expresses the SV40 T-antigen. A neomycin resistance cassette (Neor), consisting of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the herpes simplex virus thymidine kinase (HSV-TK) gene, allows stably transfected eukaryotic cells to be selected using G418. A bacterial promoter upstream of this cassette drives expression of the gene encoding kanamycin resistance in E. coli. The pEGFP-Actin backbone also provides a pUC origin of replication for propagation in E. coli and an f1 origin for single-stranded DNA production.
The pEGFP-Actin Vector expresses the EGFP-Actin fusion protein in mammalian cells. The protein is incorporated into growing actin filaments and allows for visualization of actin-containing subcellular structures in living and fixed cells. pEGFP-Actin can be transfected into mammalian cells using any standard transfection method. If required, stable transformants can be selected using G418.
载体序列
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文献和实验Restriction Map and Multiple Cloning Site of pEGFP-N2. (Unique restriction sites are in color or bold.) The Not I site follows the EGFP stop codon. The Nhe I site cannot be used for fusions since it contains an in-frame stop codon
多克隆位点区 Restriction Map and Multiple Cloning Site of pEGFP-N1. (Unique restriction sites are in color or bold.) The Not I site follows the EGFP stop codon. The Xba I site (*) is methylated in the DNA provided by CLONTECH. If you wish to digest
多克隆位点区（MCS） Restriction Map and Multiple Cloning Site of pEGFP-C2. (Unique restriction sites are in color or bold.) Note that the Eag I site is not unique.) The Xba I site cannot be used for fusions
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