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- 文献和实验
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- 库存:
100
- 供应商:
上海一基实业有限公司
- CAS号:
73087-83-9
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文献和实验P-Glucuronidase Enzyme Histochemistry on Semithin Sections of Plastic-Embedded Arabidopsis Explants
to a water-soluble mdoxyl mtermedtate that is further dimerized into a dichloro-dibromo-indigo blue precipitate by an oxidation reaction (Fig. 1 ). However, this reaction has a serious drawback. Halogen-substituted mdoxyls liberated at the site of high enzyme
deionized formamide 500 m l 10X TBE 500 m l 0.2% bromophenyl blue and 0.2% xylene cyanol Other Reagents Needed: 0.22 m M disposable syringe filters, short wave UV source, intensifying screen for UV shadowing Procedure • Pour a 20% denaturing gel: 12 ml
Protein Gel Preparation and Staining
250 mM Tris Base 1.65 g Tris Base 250 mM glycine 9.35 g glycine 0.1% SDS 2.5 ml 20% SDS up to 500 ml with Q 5X Loading Dye 0.5 M Tris 6.8 5 ml 1M Tris pH 6.8 50% glycerol 5 ml glycerol 0.5% bromophenyl blue 0.05 g
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