For highly Efficient, Accurate & Robust general Cloning from PCR High Fidelity fragments, without toxic genes use
Product Overview:
pSpark® I is a highly efficient, accurate and easy-to-use DNA cloning System based on a novel breakthrough technology to generate blunt vectors with a high blunt cloning efficiency.
The vector is prepared by digestion of pSpark® at the EcoRV site before treating both ends to prevent vector self-ligation. The end treatment is supported by a proprietary know-how that guarantees a higher cloning efficiency than with simply a desphorylated vector.
Applications
General cloning.
Cloning of High Fidelity PCR amplified products.
Production of ssDNA.
Blue/white screening for recombinants.
In vitro transcription from T7/SP6 dual-opposed promoters.
Advantages & Features
Unprecedented high cloning efficiency:more than 2,500 positive colonies expected under optimal conditions.
Easy-to-use: eliminate recombinant screening due to its minumum background (lower than 1%), avoiding “suicide” strategies from toxic genes.
Time-saving protocol:no hidden steps such as phosphorylation, just ligation after PCR and transformation.
High stability:eliminates cloning bias or pitfalls.
Powerful:clone from less than 1 ng/kb, obtain 5-fold more positive colonies using 10x less DNA insert.
Compatible with blue/white screening.
Great versatility:compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers.
Sensitive:clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert.
Eliminates positive selection vector.
High cost-saving:reduces your cloning costs as no expensive phosphorylated primers are needed.
Robust for every DNA size:just 6.7 ng per kb of insert needed for optimal ligation.