- ¥780 - 2280
- 上海雅吉生物科技有限公司
- YS-15334R
- 2025年07月08日
- WB=1:5000-20000 ELISA=1:5000-20000 IHC-P=1:500-1000 IHC-F=1:500-1000 Flow-Cyt=1μg/Test IF=1:100-1000 （石蜡切片需做抗原修复）
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic MAP peptide derived from human beta-Actin
- 亚型：
IgG
- 保存条件：
Store at -20 °C
- 克隆性：
多克隆
- 应用范围：
WB=1:5000-20000 ELISA=1:5000-20000 IHC-P=1:500-1000 IHC-F=1:500-1000 Flow-Cyt=1μg/Test IF=1:100-1000 （石蜡切片需做抗原修复）
- 浓度：
1mg/1ml
- 抗体英文名：
Biglycan
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ¥780.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ¥1380.0 |
| 规格： | 200ul | 产品价格： | ¥2280.0 |
英文名称Biglycan
中文名称骨/软骨蛋白多糖1抗体
别 名BGN; Biglycan; Biglycan proteoglycan; Bone/cartilage proteoglycan I; Dermatan sulphate proteoglycan I; DSPG1; PG S1; PG-S1; PGI; PGS1_HUMAN; SLRR1A; Small leucine rich protein 1A.
Specific References (2) | bs-7552R has been referenced in 2 publications.[IF=5.23] Han, Chao, et al. "An Animal Model of Modic Changes by Embedding Autogenous Nucleus Pulposus inside Subchondral Bone of Lumbar Vertebrae." Scientific Reports 6 (2016): 35102. WB ; Rabbit. PubMed:27713567 [IF=1.72] Huang, Guo-fu, et al. "The Effect of Electroacupuncture on the Extracellular Matrix Synthesis and Degradation in a Rabbit Model of Disc Degeneration." Evidence-Based Complementary and Alternative Medicine 2014 (2014). IHC-P ; Rabbit. PubMed:24987434
规格价格50ul 100ul 200ul
研究领域细胞生物 免疫学 信号转导 干细胞 细胞骨架 细胞外基质
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
骨/软骨蛋白多糖1抗体产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量37kDa
细胞定位细胞外基质 分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human Biglycan:101-200/368
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
May be involved in collagen fiber assembly.
Tissue specificity; Found in several connective tissues, especially in articular cartilages.
Function:
May be involved in collagen fiber assembly (By similarity).
Subunit:
Homodimer. Forms a ternary complex with MFAP2 and ELN
Subcellular Location:
Secreted, extracellular space, extracellular.
Tissue Specificity:
Found in several connective tissues, especially in articular cartilages.
Post-translational modifications:
The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate (By similarity).
Similarity:
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. Contains 12 LRR (leucine-rich) repeats.
SWISS:
P21810
Gene ID:
633
Database links:
Entrez Gene: 633 Human
Entrez Gene: 12111 Mouse
Entrez Gene: 25181 Rat
Omim: 301870 Human
SwissProt: P21810 Human
SwissProt: P28653 Mouse
SwissProt: P47853 Rat
Unigene: 821 Human
Unigene: 2608 Mouse
Unigene: 783 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
中文名称骨/软骨蛋白多糖1抗体
别 名BGN; Biglycan; Biglycan proteoglycan; Bone/cartilage proteoglycan I; Dermatan sulphate proteoglycan I; DSPG1; PG S1; PG-S1; PGI; PGS1_HUMAN; SLRR1A; Small leucine rich protein 1A.
Specific References (2) | bs-7552R has been referenced in 2 publications.[IF=5.23] Han, Chao, et al. "An Animal Model of Modic Changes by Embedding Autogenous Nucleus Pulposus inside Subchondral Bone of Lumbar Vertebrae." Scientific Reports 6 (2016): 35102. WB ; Rabbit. PubMed:27713567 [IF=1.72] Huang, Guo-fu, et al. "The Effect of Electroacupuncture on the Extracellular Matrix Synthesis and Degradation in a Rabbit Model of Disc Degeneration." Evidence-Based Complementary and Alternative Medicine 2014 (2014). IHC-P ; Rabbit. PubMed:24987434
规格价格50ul 100ul 200ul
研究领域细胞生物 免疫学 信号转导 干细胞 细胞骨架 细胞外基质
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
骨/软骨蛋白多糖1抗体产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量37kDa
细胞定位细胞外基质 分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human Biglycan:101-200/368
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
May be involved in collagen fiber assembly.
Tissue specificity; Found in several connective tissues, especially in articular cartilages.
Function:
May be involved in collagen fiber assembly (By similarity).
Subunit:
Homodimer. Forms a ternary complex with MFAP2 and ELN
Subcellular Location:
Secreted, extracellular space, extracellular.
Tissue Specificity:
Found in several connective tissues, especially in articular cartilages.
Post-translational modifications:
The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate (By similarity).
Similarity:
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. Contains 12 LRR (leucine-rich) repeats.
SWISS:
P21810
Gene ID:
633
Database links:
Entrez Gene: 633 Human
Entrez Gene: 12111 Mouse
Entrez Gene: 25181 Rat
Omim: 301870 Human
SwissProt: P21810 Human
SwissProt: P28653 Mouse
SwissProt: P47853 Rat
Unigene: 821 Human
Unigene: 2608 Mouse
Unigene: 783 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
| 产品图片 |  Sample: Bone (mouse) Lysate at 40 ug Primary: Anti- Biglycan (bs-7552R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37kD Observed band size: 52 kD  Sample: Colon (Rat) Lysate at 40 ug Embryo (Mouse) Lysate at 40 ug Primary: Anti-Biglycan (bs-7552R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 37 kD Observed band size: 52 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Biglycan) Polyclonal Antibody, Unconjugated (bs-7552R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Biglycan Polyclonal Antibody, Unconjugated(bs-7552R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: human endothelial cells;4% Paraformaldehyde-fixed; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Biglycan Polyclonal Antibody, Unconjugated(bs-7552R) 1:50, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Rhodamine B conjugated (bs-0295G-RBITC) used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei |
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文献和实验相关实验
表达Ⅱ型胶原和蛋白多糖［10］。软骨细胞在传代培 养中表型的变化可能与软骨细胞自分泌IL-1有关，而TGFβ可作为一种IL-1的拮抗剂，减 少了IL-1对胞外基质的分解代谢同时也下调了IL-1受体及其金属蛋白酶的表达［1 1］。TGFβ可能通过以下两种途径拮抗IL-1的作用，(1)刺激成软骨细胞中蛋白糖抑制 剂的产生。(2)抑制软骨细胞蛋白酶的产生，因而对细胞外基质的合成有促进作用［1 2］。 1.2骨形态发生蛋白(BMP) BMP是TGFβ的超家族成员之一，BMP-7(OP
R&D Systems:如何提高MSC的扩增且不损失其分化潜能
而制造的扩增培养基具有最大MSC扩增效率，而且不会损害MSC的多能性。 引言 骨髓间充质干细胞或者骨髓基质细胞（MSCs）是具有多种系潜能的成人干细胞，它们便于基因改造并且具有免疫抑制能力。它们用途广泛，为组织工程学，再生医学和免疫疗法带来希望。包括骨髓，骨膜，骨小梁，脂肪组织，滑膜，骨骼肌和乳牙在内的许多组织都有MSCs。它们能分化为骨，软骨，肌肉，骨髓，腱，脂肪和结缔组织。怀着对在体和体外实验深入研究的希望，我们有兴趣优化培养和扩增MSC的培养条件，同时保留MSC的多能性和分化能力
结缔组织（connective tissue）由细胞和大量细胞间质构成，结缔组织的细胞间质包括基质、细丝状的纤维和不断循环更新的组织液，具有重要功能意义。细胞散居于细胞间质内，分布无极性。广义的结缔组织,包括液状的血液、松软的固有结缔组织和较坚固的软骨与骨；一般所说的结缔组织仅指固有结缔组织而言。结缔组织在体内广泛分布，具有连接、支持、营养、保护等多种功能。 结缔组织均起源于胚胎时期的间充质（mesenchyme）。间充质由间充质细胞和大量稀薄
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骨/软骨蛋白多糖1抗体
¥780 - 2280
