- ¥1280
- BIOSS
- 北京
- YSm-0287M
- 2025年07月15日
- IHC-P=1:50-100 IHC-F=1:50-1421 （石蜡切片需做抗原修复） not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
- rabbit
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- 详细信息
- 技术资料
- 免疫原：
KLH conjugated synthetic peptide derived from human PRODH
- 亚型：
IgG
- 形态：
液态/冻干粉
- 保存条件：
Store at -2610 °C
- 克隆性：
多克隆
- 宿主：
rabbit
- 应用范围：
IHC-P=1:50-100 IHC-F=1:50-1421 （石蜡切片需做抗原修复） not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
- 浓度：
1mg/1ml
- 规格：
0.2ml/0.1ML
英文名称His tag(2D5)
中文名称小鼠抗聚组氨酸单克隆抗体
别 名HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.
文献引用：
规格价格50ul 100ul 200ul
产品类型标签抗体
研究领域细胞生物 免疫学
抗体来源Mouse
克隆类型Monoclonal
克 隆 号2D5
交叉反应 fusionprotein,HHHHHHtagged
产品应用WB=1:500-5000 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated HHHHHH peptide:HHHHHH
亚 型IgG
纯化方法affinity purified by Protein G
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
SWISS:
N/A
Gene ID:
N/A
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白
1） 6xHis与不同的Sepharose纯化填料结合（捆绑），可不受构象的影响，在天然或变形的条件下可进行进一步纯化。
2） 可在温和的洗脱液环境中，其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。
3） 6xHis标签比普通（Tag）要小得多，经纯化后的蛋白可直接进行下游操作。可用于任何表达系统（包括： 细菌，杆状病毒和哺乳动物）
4） 6xHis标签在生理溶液pH下不带电荷，其标签不会干涉重组蛋白的结构和功能， 6xHis不会影响蛋白分泌。
5） 6xHis标签免疫原性差，重组蛋白可以不必除去标签而直接作为抗原进行免疫。
中文名称小鼠抗聚组氨酸单克隆抗体
别 名HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.
文献引用：
规格价格50ul 100ul 200ul
产品类型标签抗体
研究领域细胞生物 免疫学
抗体来源Mouse
克隆类型Monoclonal
克 隆 号2D5
交叉反应 fusionprotein,HHHHHHtagged
产品应用WB=1:500-5000 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated HHHHHH peptide:HHHHHH
亚 型IgG
纯化方法affinity purified by Protein G
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
SWISS:
N/A
Gene ID:
N/A
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白
1） 6xHis与不同的Sepharose纯化填料结合（捆绑），可不受构象的影响，在天然或变形的条件下可进行进一步纯化。
2） 可在温和的洗脱液环境中，其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。
3） 6xHis标签比普通（Tag）要小得多，经纯化后的蛋白可直接进行下游操作。可用于任何表达系统（包括： 细菌，杆状病毒和哺乳动物）
4） 6xHis标签在生理溶液pH下不带电荷，其标签不会干涉重组蛋白的结构和功能， 6xHis不会影响蛋白分泌。
5） 6xHis标签免疫原性差，重组蛋白可以不必除去标签而直接作为抗原进行免疫。
| 产品图片 |  Sample: His tag protein (Human) at 100 ng His tag protein (Human) at 100 ng His tag protein (Human) at 100 ng Primary: Anti- His tag(2D5) (bsm-0287M) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 35 kD |
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小鼠抗聚组氨酸单克隆抗体
¥1280
