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- GeneAll
- 进口
- 317-106; 317-150
- 2025年12月24日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Room temperature (15~25℃)
- 英文名：
Ribospin Seed/Fruit
| Cat.No. | Product Description | Size |
| 317-106 | RibospinTM Seed / Fruit | 6 |
| 317-150 | RibospinTM Seed / Fruit | 50 |
Description
RibospinTM Seed / Fruit RNA mini kit is designed for easy and convenient isolation of total RNA from difficult plant
tissues such as seeds, fruits, and rhizomes. Especially, this kit can remove effectively large quantities of secondary
metabolites including polysaccharides and polyphenolic compounds which can lead to inhibition of downstream application.
RibospinTM Seed / Fruit RNA mini kit provides two different procedures that are available for application of various
plant tissues as follows: Protocol I for seed and fruit, Protocol II for starch-enriched grain and rhizome. For
efficient RNA purification, this kit offers optimized lysis system according to the sample type and adopts EzPureTM
filter column to eliminate impurities simply from lysate. Moreover, contamination of genomic DNA, that causes
interference in RNA analysis, can be excluded by on-column DNase I treatment in these procedures. The purified RNA
is suitable for use in various downstream procedures including cDNA synthesis, RT-PCR, or northern blotting.
Features and Benefits
Glassfiber membrane technology
■ Sample size : Up to 100 mg seed and fruit
■ Simply Removal of the impurities by using EzPureTM filter.
■ Including DNase I and treatment step
■ High purity : A260/A280 1.8~2.2, A260/A230 >2.0
■ Preparation time : ~ 30 minutes
■ No phenol / chloroform extraction
■ No ethanol precipitation
■ Ready for use in RT-PCR, northern blotting, dot blotting, in vitro translation,
molecular cloning, real-time PCR, RNase protection assay and other
analytical procedures
Procedure
RNA Purification Result
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文献和实验Tomato (Lycopersicum esculentum)
, particularly for oral delivery; for many of the same reasons, it is a popular vegetable. Its fruit does not contain toxic substances and is palatable uncooked; it is easily processed; the plants are able to be propagated by seed or clonally by tip or shoot
is found to be 2.5x106 cells/ml. You wish to seed the 5 dishes at a density of 7x105 cells/dish, and in order to promote good gas exchange in the dishes you have decided to limit the depth of the medium in the dishes to 6mm (0.6cm). The area of the dish bottom is 28
is found to be 2.5x106 cells/ml. You wish to seed the 5 dishes at a density of 7x105 cells/dish, and in order to promote good gas exchange in the dishes you have decided to limit the depth of the medium in the dishes to 6mm (0.6cm). The area of the dish bottom is 28
