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- 2025年12月24日
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- 英文名：
RiboEx LS Total RNA
| Cat.No. | Product Description | Size |
| 302-010 | RiboExTM LS | 10 ml |
| 302-001 | RiboExTM LS | 100 ml |
| 302-002 | RiboExTM LS | 200 ml |
Description
RiboExTM LS is a complete kit with ready-to-use reagents for the isolation of total RNA from various liquid samples. RiboExTM LS is a concentrated form of RiboExTM and this allows that liquid samples can be processed more easily with it, while RiboExTM is more suitable for solid samples and pelleted cells. RiboExTM LS is a mono-phasic solution containing phenol and guanidine salt, which rapidly lyse cells and inactivates nucleases. Addition of chloroform brings about a separation of the homogenate in aqueous and organic phases. RNA locates in the aqueous phase while DNA and protein remain in the interphase and organic phases. The aqueous phase including RNA is mixed with isopropanol and the RNA which is precipitated by centrifuging. The purified total RNA is suitable for RT-PCR, northern blotting, dot blotting, in vitro translation, molecular cloning, realtime PCR, RNase protection assays and other analytical procedures.Features and Benefits
■ Format : Monophase solution type
■ Sample size : Up to 0.25 ml liquid sample / 0.75 ml RiboExTM LS
Up to 100 mg tissue / 0.75 ml RiboExTM LS
■ Preparation time : 50 ~ 65 minutes
■ Typical yield : Up to 30 ㎍ / 1 x 106 cultured cells
Up to 10 ㎍ / 1 mg tissue
■ High purity : A260/A230 > 2.0, A260/A280 > 1.8
■ Accurate and consistence yield
■ Ready for use in RT-PCR, northern blotting, dot blotting, in vitro translation, molecular cloning,
real-time PCR, RNase protection assays and other analytical procedures
Procedure
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文献和实验Isolation of Total RNA from Difficult Tissues
The often exacting process of isolating intact total RNA from tissue becomes even more difficult when processing certain problematic tissues. Fibrous tissues and tissues rich in protein, DNA and nucleases present distinct
. It is therefore prudent to investigate expression at the RNA and protein levels. This chapter will discuss the isolation of total RNA from yeast. The reader should refer to ref. (1 ) for a detailed description of the methods for preparation of ribosomal and small RNAs
DNase I Treatment Of Total RNA
（1 U/ul）1 ul 10X DNaseI bufferDEPC water to 10 ulExample of a scaled up version for a 40 ul reaction100 ug Total RNA4 ul DNase I （1 U/ul）4 ul 10X DNaseI bufferDEPC water to 40 ulPrepare your reaction and mix well.Incubate 37°C for 30 minutes. Stop
