Recombinant Trichosanthes kiri

Bispecific Monoclonal Antibodies for the Targeting of Type I Ribosome-Inactivating Proteins Against Hematological Malignancies

for the generation of ITs, although several other single-chain ribosome-inactivating proteins (RIPs) or type I RIPs are also available (3 ). Type I RIPs are RNA N -glycosidases displaying potent inhibition of protein synthesis in cell-free assays, but they lack

Ribosome Display and Screening for Protein Therapeutics

Ribosome display is a cell-free display technology which enables in vitro selection of antibodies from large recombinant DNA libraries. It also allows continuous introduction of mutations into the selected DNA pool by PCR-based mutagenesis

Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting

Basic Protocol 1: Generation of an Active Recombinant Protein That is End‐Labeled Basic Protocol 2: Hydroxyl Radical Cleavage of Protein in Absence and Presence of Ligand Basic Protocol 3: Analyze Gel