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文献和实验Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
(most critical timed step). 5. Add 300 ul of cold Qiagen buffer R3 (neutralization buffer) per well. Seal wells with plastic sealing tape and mix by gentle inversion 5 times. Incubate on ice for 5 minutes. 6. Add 50 ul of ProCipitate™ (LigoChem
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
(most critical timed step). 5. Add 300 ul of cold Qiagen buffer R3 (neutralization buffer) per well. Seal wells with plastic sealing tape and mix by gentle inversion 5 times. Incubate on ice for 5 minutes. 6. Add 50 ul of ProCipitate™ (LigoChem
Mag-Bind Plant RNA Protocol using 96-well Plate
by vortexing at maximum speed for 30-60 seconds. 3. Add 100 uL Buffer SP2 and seal the plate with Sealing film vortex to mix throughly. Note: It is critical to mix the sample throughly by vortexing vigorously to give optimized yield.
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