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上海希言科学仪器有限公司
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文献和实验the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
BrdU-Immunoperoxidase Staining of Paraffin Tissue Sections
horse anti-mouse IgG) 1/250, 30' (x1). Wash (x3) 11) 100 µL Avidin + Biotin-HRP mix (1/125 v/v Sol.A, 1/125 Sol B), 30' (x1). Wash (x3) In Coplin jars (25 mL): 12) 50 mM Tris pH8 37°C (x1)
Analysis of Proteins using Small Format 2D Gel Electrophoresis
dithiothreitol, 2.3% (w/v) SDS) for 7 minutes before embedding in 1% (w/v) agarose prepared in 0.125M Tris-HCl, pH 6.7, 0.1% (w/v) SDS with bromophenol blue to colour. The second dimension slab gels (70 x 80 mm) are prepared in batches of 12 gels using
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