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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
Embryonic Fibroblasts (Irradiated) 4. DMEM with GlutaMAX™-I (High Glucose) 5. KnockOut™ DMEM/F-12 6. Fetal Bovine Serum (FBS), ES Cell-Qualified 7. KnockOut™ Serum Replacement (KSR) 8. MEM Non-Essential Amino Acids (NEAA)
Large Scale Plasmid Preps: Qiagen/Cesium Method
). Heat seal the centrifuge tube. Spin for 4 hours in a Ti65 vertical rotor at 65,000 RPM, at 20ºC, no brake, or overnight at 55K RPM. Remove the lower (supercoiled DNA) band through a syringe and needle with UV illumination. Using UV
with rubber stopper and vaccum adapter vacuum source funnel & Whatman filter paper BIORAD sequencing gel apparatus beakers (50 mL and 100 mL) syringe (60 mL) with large needle styrofoam box (inclined) styrofoam box (rectangular) gel clamps
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