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- 2025年07月16日
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文献和实验Clonality - X Chromosome Inactivation Assay
of the resuspended DNA into a reaction tube. Add 1 µl REact buffer 2. Add 1 µl Hha I. Incubate overnight at 37°C. Incubate at 90°C for 10 min. Use 2 µl of this digested DNA for PCR (see below). Use 2 µl of the non-digested DNA from B18 above as a negative
and the supernatant discarded) twice with Solution A (1.2 M sorbitol, 50 mM KPO4, pH 7). To spheroplast, cells are resupended in 500 ul of Solution A containing 0.1 b-mercaptoethanol, 0.02 glusulase and 5ug/ml zymolyase. The suspension is incubated at 37oC
of 0.75 to 1. To fix, 1/10 volume of 37% formaldehyde is added to the culture, which is shaken for a further 40 minutes at room temperature. Cells are then recovered by centrifugation at 1400 X g for 2 minutes, and washed (i.e. gently resuspended
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