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文献和实验Isolate DNA from NRBC Blood from Buccal Swab or dried blood spots collected with filter paper
,000 x g for 10 minutes. Carefully transfer the supernatant to a new 1.5 ml tube. 5. Add equal volume of absolute ethanol (room temperature, 96-100%) to the sample and mix by vortexing at maxi speed for 15 seconds. Collect any liquid drop from lid
Hybridization of High Density Arrayed BAC Nylon Filter Blots
. Immediately incubate probe 65°C, 2hrs to preanneal. Prewet filter blots in dH2 O tray. Roll a maximum of 3 high density filter blots together and place into roller hybridization bottle (Robbins Scientific Inc). Add 25ml of 65°C
medium over the roots and shake the filter unit vigorously in the solution. Repeat rinse with a clean petri dish until liquid is clear. Blot the roots on filter paper. Transfer root bundles to MSg v500 containing 50 mg/l kanamycin, 58 bundles per 25 x 100
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