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文献和实验Flow Cytometric Measurement of Cell Proliferation
The simplest guide to cell proliferation that can be obtained by the use of flow cytometry is the S-phase fraction (SPF) calculated from DNA histograms. Measurement of such histograms was one of the earliest applications of flow cytometry
Flow Cytometric Analysis of Cell Cycle
Flow Cytometric Analysis of Cell Cycle Contributor: Suprya Jayadev Date: July 13, 1994 Fixation 1) Collect 2 X 106 cells. 2) Pellet cells by spinning at 1,000 rpm, 4°C for 5 minutes
at 1000rpm (300xg) For 96 well plate, wash cells three times with 150ul L-15 Resuspend stained cell pellets in 400ul L-15 at 4o C for flow cytomery Cells can be resuspended in fixation solution and stored for 1 week (optional) Add 10
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