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文献和实验Gibson Assembly is a high-efficiency DNA end-linking technique developed by Daniel Gibson at the JCVI in 2009 [Gibson et al. 2009 plus supplementary methods]. The technique was invented and perfected as part of the genome assembly efforts at JCVI
CSF Extract Prep for Spindle Assembly
that we find are most important for obtaining good CSF extracts that are competent for CSF spindle assembly and for cycled spindle assembly. The indicated buffer amounts are sufficient for a 4 frog prep. For sperm nucleus preparation, see above protocol. For spindle assembly
Chromatin Assembly Using Drosophila Systems
Literature Cited Banerjee, S. and Cantor, C.R. 1990. Nucleosome assembly of simian virus 40 DNA in a mammalian cell extract. Mol. Cell. Biol. 10:2863‐2873
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