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- 2026年02月18日
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Liquid nitrogen feed tube assy., Exchange assy, 1 assy. Rebuilt of MJ1-1303
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文献和实验Freezing cells in liquid nitrogen
Take off Media Trypsinate with 1ml x2 Dulbecco A trypsin Add 7ml Media Pipette up and down to distribute cells throughout media (i.e. not clumped together) Add media to sterile falcon tube (15ml = 1 flask, 50
Tissue Culture - Thawing Cells from Liquid Nitrogen
Tissue Culture - Thawing Cells from Liquid Nitrogen METHOD: Prepare before thawing: Fill a test tube of 10 - 15 mls of cold media (appropriate for your cell line) with 10 - 20% fetal calf serum (use 2x the % you use
0.01% boric acid 1 mM MgSO4 2mM CaCl2 --this was used with media containing GABA. If no other nitrogen source is present, use 1mM CaCl2 and 1 mM CaNO3 18% sucrose pH 6.5 Air dry flowers for 1-2 hours (optional--this helps
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