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文献和实验Immunofluorescent Localization of Tubulin
Rabbit anti-tubulin (or other primary antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter
Pollen Development in Anthers of Arabidopsis
apart from the anthers. 10. Carefully remove excess Acetic Acid with a piece of filter paper and immediately add a drop of 1% Propionic Carmine to stain the chromosomes (see Hint #1). 11. Gently tap the preparation with a copper rod
Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber)
in the wells, then drop the ends. Once you have dropped the filter, do not move it around. 5. Carefully place the gasket on top of the filter and then the top portion of the chamber apparatus. 6. Secure the apparatus with the screws provided
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