Filter (last drop) with 2um Pa

Isolate DNA from NRBC Blood from Buccal Swab or dried blood spots collected with filter paper

mg/ml) into the sample. 2. Add 25ul OB Protease and 500ul Buffer BL into sample. Mix by vortexing at maxi speed for 30s. 3. Incubate at 65°C for 10 minutes. Collect any liquid drop from lid by brief centrifugation. 4. Centrifuge at 14

FACSAria流式细胞仪无菌分选的基本流程

。注意速率不要超过4。 8、点击打开Voltage，点击sort按钮，点击Optical Filter，调节drop delay，使屏幕左边的方框中的数值为98％ 以上，即调整好了drop delay。Drop delay是影响分选质和量的重要指标，一定要调整好。 通过上述操作即完成了全部的分选前的环境消毒和某些参数的调试工作，后续和分析无较大差别。 在整个的无菌分选过程中，要时刻注意保持无菌的环境，注意观察液流的形态和位置。当液流出现异常时，一定要停止分选，及时的调整，因为正确的液流形态和位置

Cytokine induced angiogenesis in chick embryos.

a pencil. Open the blunt end of the egg with forceps. Peel back the shell membrane, being careful not to damage the CAM membrane.     4. Drop the filter paper over the embryo in the area with the least number of visible blood