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- 2025年07月16日
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文献和实验Clonality - X Chromosome Inactivation Assay
. Pipet upper phase into new tube and discard lower phase. Add 5 µl 3M NaAc. Add 250 µl isopropanol. Add 2 µl glycogen. Vortex briefly. Place on dry ice for 30 min. Centrifuge 14,000 rpm for 30 min. Add 300 µl 70% ethanol. Vortex briefly
Fast and reliable mini-prep RNA extraction from Neurospora crassa
Eppendorf tube. The tube can be filled with powdered mycelium - Shake for 15-20 min (Eppendorf Rotationsmischer 3300) - Centrifuge for 10 min, 10,000 rpm - Transfer the upper phase into an equal volume of phenol (saturated with 0.1 M Tris HCl, pH
A quick RNA mini-prep for Neurospora mycelial cultures
15. Spin 10-20 min in microfuge. Wash twice with 70 EtOH and dry pellet. Resuspend in 200 µl of sterile RNase-free gd H2O. Store at -70°C for up to three months. Spectophotometric quantification may be done at this point. 16. Load 1 µl
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