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文献和实验DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
is precipitated by adding Protein Precipitation Reagent (PPR). After removal of the protein, the supernatant is mixed with 1 volume of isopropanol to precipitate the RNA. After the centrifugation to pellet the RNA, the supernatant is transfer to a new tube
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
-galactopyranoside (X-gal; 25 mg/mL in DMF [dimethylformamide]) Agarose gel (1%, UltraPure; Invitrogen) Antibody, specific for the protein of interest Bead eluting buffer Bovine serum albumin (BSA; 0.5% in PBS; New England Biolabs) Cell
. The phage particle are precipitated with PEG, collected by centrifugation, and the pellet is resuspended in buffer. The phage protein coat is denatured and removed by one phenol and two ether extractions. After ethanol precipitation, the dried DNA pellet
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