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- 2025年07月11日
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文献和实验so that a 75 x 25 x 1 mm microscope glass slide will sit flush in the chamber. 7) Create a recess for an O-ring around the underside of the top and bottom plates such that they are spaced apart by 0.02". Insert the rubber O-rings. 8)
RLCS (Restriction Landmark cDNA Scanning) Protocol
2 mL collection tube. To bind DNA, apply the sample to QIAquick column and centrifuge (13,000 rpm) for 30-60 sec. Dicard flow-through. Place the QIAquick column back in the same tube. To wash, add 0.75 mL buffer PE to the column
. Have the tubing and connections ready to connect the column in tandem with a G25 column (2.5 x 44 cm), also equilibrated in Buffer G, but don''t have them connected yet. The tubing between the outflow of the DNaseI column and the inlet of the G25 column
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