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上海希言科学仪器有限公司
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文献和实验Construction and Manipulation of Large-Insert Bacterial Clone Libraries
. II. Reagents 10 x homogenization buffer (HB) stock: 0.1 M Trizma base, 0.8 M KCl, 0.1 M EDTA, 10 mM
the CRC filter (35-µm) into the CRC. Place the CRC in a standard microcentrifuge tube. Add ~30 µL of the Ni-NTA resin slurry to the bottom of the CRC tube. Drain the column by centrifugation at 120g for 1 min at room temperature. These centrifugation
10 µl Bio-Rad Kaleidoscope Prestained Standards #161-0324 directly. 3. Run with constant voltage (120V) 4. Usual running time is about 1:40 F. Preparation of membrane 1. Cut a piece of PVDF membrane(Pall Corporation ). 2. Wet for about 30 min
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