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文献和实验Whole genome amplification protocol for ChIP-chip
to a genomic tiling array. Therefore, we have adapted the standard protocol for whole genome amplification using the Sigma GenomePlex WGA kit to amplify our ChIP sample (O'Geen et al., 2006). Using Oct4 ChIP-chip assays as an example, we have compared
Plant DNA methylation analysis by bisulfite genomic sequencing
DNA clean up resin (Promega kit #A7280), vortex (see comment 4); Load resin on provided column, apply vacuum (vacuum flask, membrane pump); Add 2.0ml 80% (v/v) 2-propanol on column, apply vacuum (vacuum flask, membrane pump);
-2-phenylindole (DAPI, Cat. No. D-8417), and diethylpyrocarbonate (DEPC, Cat. No. D-5758) were obtained from Sigma. The glass plates used for hybridization (Cat. No. 165-1824) were obtained from Bio Rad. The ProLong Antifade Kit for mounting slides (Cat. No. P
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