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上海希言科学仪器有限公司
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文献和实验Nano-LC Coupled to MS/MS . In summary: i. Pull a fused-silica capillary microcolumn (100 µm I.D. x 365 µm O.D.) with a P-2000 laser puller. ii. Pack the microcolumn with 10 cm of C18 reversed-phase material
Synthesis of Peptide‐Oligonucleotide Conjugates by Diels‐Alder Cycloaddition in Water
Synthesis columns for 1‐µmol scale synthesis (Applied Biosystems or Glen Research), controlled‐pore glass (CPG, 500 Å) 4‐mL screw‐cap pressure tubes, O‐ring seal preferred
in water gradient, both containing 0.1% (v/v) TFA, at 1 mL/min over 30 minutes at 60 °C with a C18, 5 μm, 50 X 2 mm column) and MALDI (Fig. 5). Purification with reverse phase HPLC on a Vydac C18 column (10 μm, 22 mm x 250 mm) proceeded, using
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