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- 2025年07月09日
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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
the medium, and re-suspend the cells in an appropriate amount of fibroblast medium. d. Count the cells using the desired method (e.g., Countess® Automated Cell Counter), and seed the MEF culture dishes with 5 × 104 –2 × 105 cells per 100-mm dish
Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period
mixture to cool to room temperature before finally correcting pH. Make up to 1 liter with distilled water. Autoclave at 15 p.s.i. for 15 min. Preparation of Red blood cell lysis buffer: 0.01 M Tris-HCl pH 7.6, 320 mM sucrose, 5 mM MgC12, 1% Triton X
RNA‐Seq: A Method for Comprehensive Transcriptome Analysis
) 100 mM dithiothreitol (DTT) 200 U/µl SuperScript II Reverse Transcriptase (Invitrogen) 5× second
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