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文献和实验an automated cell counter (Vi-Cell® XR analyzer, Cat no. 383556; Beckman Coulter, Fullerton, CA, USA) and Vi-CELL® XR Quad Pak Reagent Kit (Cat no. 383198; Beckman-Coulter, Fullerton, CA, USA). The automated cell counter mixes the cell suspension with an equal
Isolate It All：siRNA • miRNA • Total RNA • Native Protein
expression levels from the same experimental sample. Two Kits in One To isolate protein, small RNA and long RNA species from the same sample, Ambion scientists have developed a unique tool: the mir Vana™ PARIS™ Kit . Quantitative recovery of small
RNase and DEPC Treatment: Fact or Laboratory Myth
. Figure 1. Effect of Autoclaving on RNase Activity. Various concentrations of RNase A were added to PBS and autoclaved for 25 minutes. 1 µl of each solution was mixed with 1 ng of a 5 x 104 cpm RNA probe which was 304 bases long and incubated
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