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- 美国沃特世Waters
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- 186005225
- 2025年07月14日
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上海希言科学仪器有限公司
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文献和实验PCR purification kit) purify DNA through Qiaquick column elute DNA into 50 µl H2 O use 0.5 - 1 µl per 25 µl PCR reaction: 1 x 95 °C, 2 min 21 x 95 °C, 30 sec; 60 °C, 30 sec; 72 °C, 1 min 1 x 72
Purification of recombinant sBRF M166L
culture with 0.4 mM IPTG (4 ml of 0.1 M IPTG per 1000 ml) and grow at 30 deg. for four hours. Take a small sample of the induced and uninduced cultures and save for analysis of whole cell protein by SDS PAGE. Harvest cells by centrifugation (4-6g
PCR purification kit) purify DNA through Qiaquick column elute DNA into 50 µl H2 O use 0.5 - 1 µl per 25 µl PCR reaction: 1 x 95 °C, 2 min 21 x 95 °C, 30 sec; 60 °C, 30 sec; 72 °C, 1 min 1 x 72
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