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文献和实验Phosphoamino acid analysis:Mark Kamps's method
your sample.We use 0.1 mm EM cellulose plates You can spot the whole sample if you are skillful and have no choice because you don't have many counts. Spot 0.25-0.30 µl at a time and dry with house air,blown through a plugged pipet,between applications
Method for Single-Cell Electroporation
-10 µm Eppindorf Pipettor tip, or a 1 cc plastic insulin syringe that had been melted over flame and pulled to a long fine tip. Circuit setup: A thin silver wire (diameter 0.25mm) is inserted into the micropipette touching the DNA solution
Phosphoamino acid analysis:Mark Kamps method
Phosphoamino acid analysis:Mark Kamps's method 1. Label your protein with 32Pi. Then subject the protein to SDS polyacrylamide gel electrophoresis and transfer your gel-fractionated protein to Immobilon-P .
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