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上海希言科学仪器有限公司
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文献和实验Anti-DYKDDDDK tag (L5) Affinity Gel
, change buffer into PBS (PH7.2) and filter the protein sample by a 0.45 or 0.22 µm filter to be ready for L5 purification. 2. Purifying DYKDDDDK-tag fusion proteins from mammalian cells 1) Adherent cells: trypsinize cells.Suspension
Small scale His-Tag fusion protein purification under denaturative conditions
) Dnase 100U/ml or 25-50ug/ml (SIGMA DN-25). Incubate 10min 4°C in the presence of 10mMMgCl2 Equilibration buffer: 6 to8M Urea, 50mM Na2 HPO4 pH 8.0, 0.5M NaCl Washing buffer: 6 to8M Urea , 50mM Na2 HPO4 pH 8.0, 0.5M NaCl Elution
Digital Gene Expression by Tag Sequencing on the Illumina Genome Analyzer
100% and ice‐cold 70% ethanol 1.5 µM GEX Adapter 2 (from Illumina Tag Profiling Sample Prep Kit, cat. no. FC‐102‐1005
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