AccQ-Tag Column, 60Å, 4 µm, 3.

Column Buffers: CREB

of buffers prior to use. Storage Buffer for Column For 500ml 10mM Tris ph 7.5 5ml of 1M 0.3M NaCl150ml 1M or 60ml 2.5M 1mM EDTA 1ml of 0.5M 0.02% NaN3 1ml of 10% CREB Column - Purification Trial #1 Sample #5-C4-1-1 (pg.25)lysed in 4.5ml (3 vol

Anti-DYKDDDDK tag (L5) Affinity Gel

实验试剂   Column equilibration buffer: 1xPBS/0.5M NaCl   High salt wash buffer: 50mM Tris 8.0/2M NaCl   Elution buffer: 200mM Glycine/150mM NaCl, PH2.2   Neutralization

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

at �80°C. (14) Aliquot 100 µl of the supernatants into 1.5 ml microfuge tubes, and add 900 µl of 150 mM NaCl ChIP Dilution buffer/1x proteinase inhibitors. This dilution decreases the SDS concentration in chromatin samples