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文献和实验of 7 x 2 x 0.5" and bottom dimensions of 7 x 2 x 0.25". 2) Recess the top plate in the center portion to 0.125" deep, leaving 0.950" per end for 1.375" wide x 0.3125" deep fluid reservoir. 3) Drill 1/16" wide x 0.625" long
Construction and Characterization of Adenovirus Vectors
The lysate should have a thick, highly viscous consistency. 36. Add 0.3 mL of 2 M MgCl2 , 0.15 mL of 10 mg/mL RNase A, and 0.15 mL of 10 mg/mL DNase I (Ad). Incubate with occasional inversion for 30-60 min at 37°C. 37
6. Centrifuge the block in the GPR centrifuge at 3000 rpm at 4degC for 30 minutes. 7. Carefully remove 200 ul of the supernatant from each well in the 96 well block with the 12 channel pipetter and transfer them to a v-bottom microtiter plate, being careful
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