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- 2025年07月08日
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文献和实验Genotyping using Affymetrix arrays
) in 100 µL volumes containing 1.0µM of primer (5’-ATT ATG AGC ACG ACA GAC GCC TGA TCT-3’), 0.30 mM dNTPs, 1.0 mM MgSO4, 5 U Platinum® Pfx Polymerase (Invitrogen), PCR Enhancer (Invitrogen) and Pfx Amplification Buffer (Invitrogen). 30 cycles of PCRs
Geno typing using Affymetrix arrays
for 30 seconds, and 68 °C extension for 60 seconds. As a check, 3 µL of PCR products are visualized on 2% TBE agarose gels to confirm the size range of amplicons. The PCR products are purified over MinElute 96 UF PCR Purification plates (Qiagen
denaturation for 15 seconds, 60 ℃ annealing for 30 seconds, and 68 ℃ extension for 60 seconds. As a check, 3 µL of PCR products are visualized on 2% TBE agarose gels to confirm the size range of amplicons. The PCR products are purified over MinElute 96 UF PCR
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