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文献和实验. 20X SSC Stock Solution Add 175.3g NaCl and 88.2g sodium citrate to 800 ml distilled water and pH to 7.0. Add water to 1000 ml and autoclave to sterilize. Removing Probe From Nylon Membranes 1. If reprobing is desired, do not allow the Zeta-Probe
of isolation of nucleoli of the Walker carcinosarcoma and liver of the rat. Cancer Res. 1963; 25:693-697). 1. Seed HeLa cells (ATCC number: CCL-2) on to 10x14 cm Petri dishes and culture at 37oC in 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM
buffer for SDS-proteins using nitrocellulose (with methanol) or Zeta-Probe membrane (without methanol):25 mM Tris, 192 mM glycine (20% methanol), pH 8.3 Dissolve 3.03 g Tris and 14.4 g glycine in ddH2O (add 200 ml of methanol); adjust volume to 1 liter
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