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上海希言科学仪器有限公司
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文献和实验Mag-Bind Plant RNA Protocol using 96-well Plate
-01) suitable for 96-well microplate to magnetize the Mag-Bind particles. 10. Wait until the magnetic beads form a pellet in the side of the wells (if using MSD-01) or until the solution is clear(if using another magnet). Aspirate and discard
Tissue culture treated 6‐well and 96‐well flat bottom plates (BD Falcon, cat. nos. 353046 and 353072) 15‐ml conical centrifuge tubes (BD Biosciences, cat. no. 352097
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
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