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- 2025年07月13日
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上海希言科学仪器有限公司
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24个/箱
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文献和实验ES / MEF cell culture and electroporation of targeting construct
in case of contamination or poor monolayer.Sunday, Day 5Thaw ES cells as follows: Suction the existing MEF media off one of the inactivated MEF 10 cm dishes. Refeed the dish with 12 ml ES media. Thaw one frozen vial of RW4 cells (1x106 cells) in a 37oC
ES / MEF cell culture and electroporation of targeting construct
inactivated MEFs in 2-10 cm dishes having a final concentration of 1x106 Mef's per dish and a final volume of 12.0 ml. We prepare 1 extra 10cm dish in case of contamination or poor monolayer. Sunday, Day 5 Thaw ES cells as follows: Suction
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
as described in Steps 13-15). Transfer one sphere (from Step 12) to each well using a P200 pipette and incubate overnight at 37°C. 17. After 24 h, ensure spheres have attached to the slide (Fig. 3B ); then carefully remove medium. Add 200 µL
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