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文献和实验CO2 Incubator Mechanical Pipettor Inverted microscope Vacuum pump and flask Materials Complete cell culture medium, appropriate for the cell line Tissue culture flasks of appropriate sizes Tissue culture plates - 96
amount in your new containers . For our lab, using PtK1 cells, the standard distribution is in a 1 to 3 or 4 ratio for a new flask and 5-9 drops for a coverslip dish. Again this ratio will vary with your cell line. Use of ciprofloxacin-treated
ES Cell Culture and Manipulation
for 2 minutes at 1000 rpm Aspirate off media and resuspend cell pellet in fresh media, plate Growing cells Grow cells in 10 cm tissue culture dishes Change media every 2 days Split
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