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- Kingfisher Biotech
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- KPB1061U-050
- 2025年07月12日
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文献和实验Immunohistochemisty-Fluorescence Protocol
Materials Cytokine-specific Primary Antibodies unlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems ''AF'' or ''BAF'' series) or selected monoclonal antibodies. Secondary Antibodies
ELISPOT (Enzyme-linked ImmunoSPOT)
by the coated antibody on the high surface area PVDF membrane. The wells are washed to remove cells, debris, and media components. A second antibody (biotinylated) reactive with a distinct epitope of the target cytokine is employed to detect the captured
Immunohistochemistry: Fluorescence Protocol 2.0
that have been harvested, fixed and permeabilized at the peak of cytokine production after in vitro stimulation with strong polyclonal activators such as phorbol 12-myristate 13 acetate/ionomycin, anti-CD3+ CD28 monoclonal antibody, LPS or bacterial superantigens
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