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- American Research Products
- 23-B0036PF-25 ug
- 2025年07月16日
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25 ug
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文献和实验without shaking and checked periodically. As soon as the settled cell pellet loses its creamy, yellow colour and becomes translucent (30 to 45 mins), cells should be recovered and washed twice with Solution A. Cells are resuspended in 200 ul of solution
).8. Block with 4% BSA in TBST for 45 - 60 minutes. I use RIA grade BSA from USB (United States Biochemical; Catalog number 10868). I also include 0.02% NaN3 in this block and reuse it at least 5 times (Keep in 4oC between uses). We have had problems
Anti-HA Immunocytology The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots
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