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文献和实验renaturation temperature 最适复性温度 OT oxytocin 催产素 PAG protein A-gold technique 蛋白A-金技术 PAP perbxidase anti-peroxidase complex 过氧化物酶-抗氧化物酶复合物 PB phosphate buffer 磷酸缓冲液 PBS phosphate buffered saline 磷酸盐缓冲液 PCA gastric parietal cell
Sequential RNA and DNA fluorescence in situ hybridization
with 1XSSC 3 times for 5 minutes each at 60 °C; Incubate with 1% H2 O2 in PBS for 30 minutes at RT (comment 1); Block with 3% BSA/ 2XSSC/ 0.1% Tween 20 for 15 minutes at RT; Incubate with a fluorophore-conjugated anti-DIG antibody diluted
of each fragment was normalized and expressed as percentage of the highest value. Binding data was represented in a color gradient (higher binding corresponds to darker boxes). (C) DNA-binding ELISA of VE-1, VE-5, and VE-8 proteins with the DNA duplex pr�88
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