Anti-Cyclin D2 Antibody

Immunodetection of cyclin D1 and D2/D3 using flow cytometry

that has been kept -20°C. Incubate the cells for at least two hours. Wash once with staining buffer and decant. Add the primary anti-cyclin antibody in a volume of 200 µ l. Incubate overnight at 4°C. Wash twice with cold cyclin staining buffer and add the Cy

Human Anti-antibody Response

a simple ELISA method to detect human anti-antibody response.

Purification Of Anti Peptide Antibody

antibody that could contribute to staining background. The proportion in each pool varies with the peptide immunogen. The quality of the anti-peptide serum seems to increase with multiple boosts - the first bleed may be feeble.