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文献和实验Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells
This chapter describes the analysis of long- and short-lived plasma cells on tissue sections. Mice are immunized with 4-hydroxy-3-nitrophenyl acetyl (NP) coupled to a T-cell-dependent carrier. Antigen-specific germinal center cells
to analyze very large numbers of cells, and results can be obtained within a few hours of the specimen being taken. The methods described allow B-cell lymphomas to be crudely divided into two groups, those with a germinal center phenotype
T-Cell-Dependent Immune Responses, Germinal Center Development, and the Analysis of V-Gene Sequences
in single GC B cells directly dissected from frozen tissue sections. The V Ox1 L-chain sequences are amplified by polymerase chain reaction (PCR), cloned, and sequenced. Methods are described to analyze V-gene sequences. Potential difficulties
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