IHC image of Histone H1.0 staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab11080, 7.5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97240, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
[1]Erin N. Scully,Juliana Montoya Sanchez,Christopher B. Sturdy. Abcam Monoclonal Egr-1 ab133695 is an effective primary antibody replacement for Santa Cruz sc-189 polyclonal Egr-1 in songbirds[J]. Heliyon,2019,5(11).