- ￥6378
- Agrisera
- 瑞典
- AS16 3715
- 2026年02月19日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
Goat anti-Mouse IgG1 (subclass specific), HRP conjugated
- 规格：
1ml
| Immunogen Pools of purified homogenous IgG1 isolated from pooled mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
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Host Goat
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Clonality Polyclonal
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Purity Purified goat IgG
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Format Lyophilized
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Quantity 1 ml
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Reconstitution For reconstitution add 1 ml of sterile water. Let it stand for 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage The lyophilized conjugate is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and Stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
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Tested applications Dot blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)
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Recommended dilution 1 : 1000-1 : 10 000 (ELISA), 1 : 100-1 : 500 (IHC)
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名称：Goat anti-Mouse IgG1 (subclass specific), HRP conjugated
货号：AS16 3715
品牌：Agrisera
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文献和实验Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
. A cheap alternative is to exploit the relative lower levels of each IgG isotype in serum and on follicular B cells to stain with isotype-specific, conjugated, anti-mouse secondary antibodies. IgG isotypes account each for 20-25% ot total serum
: Total mouse IgG Ig Type: mouse IgG specific goat IgG Applications: Western blot ELISA Preparation Produced in goats immunized with purified total mouse IgG. Mouse IgG1, mouse IgG2A, mouse IgG2B and mouse IgG3 specific IgG was purified by mouse IgG
T-cell dependent antigen specific in situ staining (Pe)
HRP-conjugated secondary Ab choice: 10- add the HRP-conjugated Goat anti rabbit (Vector 50 to 100 µl, dilution 1:100) and incubate for 30’. Swine anti-rabbit (Dako) can be used at 1:300 dilution. If you use this step for tyramide
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