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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
冷藏
- 保质期:
长期
- 库存:
大量
- 供应商:
康朗生物
- 规格:
150ul
2×ligation solution MasterMix
编号:KL108规格:150ul
● 保存:-20℃
● 产品简介:
2×ligation solution MasterMix是含有T4 DNA Ligase,ligation buffer的2×MasterMix,实验时,只需加入片段和载体即可进行连接反应。
适用于DNA*(代"片")段和载体DNA的连接以及DNA*(代"片")段和Linker或Adaptor DNA的连接。
● 注意事项
1.2×ligation solution MasterMix由于含有T4 DNA ligase,使用前冰浴中彻底融化,混匀后使用。
2.为了提高转化效率,转化时建议所加入连接产物的量不要超过感受态细胞体积的10%。
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文献和实验Linker Ligation (with T4 ) DNA Ligase In a microcentrifuge tube prepare a solution of blunt ended, dephosphorylated DNA (100-500ng) in TE buffer (5-7µl). Add 1-2µg of phosphorylated linkers in 5µl of TE buffer.
Linker Ligation (with T4 ) DNA Ligase In a microcentrifuge tube prepare a solution of blunt ended, dephosphorylated DNA (100-500ng) in TE buffer (5-7µl). Add 1-2µg of phosphorylated linkers in 5µl of TE buffer. Add: 10X ligation buffer 2µl
have been suggested to be able to stick to the ends of thereby affecting ligation. There is uncertainty if this is the case, or if it is a problem of enzyme carrying over during purification. In such cases the solution needs to be treated with proteinase K followed
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