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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
Histone H3 (Phospho-Ser28) Antibody HRP Conjugated
- 靶点:
Histone H3 Ser28
- 浓度:
1mg ml
- 应用范围:
WB, IHC-P
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat
- 标记物:
HRP
- 克隆性:
Polyclonal
- 保存条件:
Store at 4°C for 12 months.
- 形态:
liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser28
- 规格:
100ul
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文献和实验Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
Growth factor stimulation results in phosphorylation of histone H3 at ser 10 and this correlated with expression of immediate early genes suggesting that this phosphorylation is associated with transcriptional activation. Although Western
FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells
scanner (FACS) is described, based on the presence of an intranuclear antigen present only in mitotic cells, detected using a specific, commercially available antibody. Cell staining and FACS analysis can be done in a single day, making this a rapid
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
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