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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
AKT1 2 3 (Phospho-Ser472 Ser473 Ser474) Antibody HRP Conjugated
- 靶点:
AKT1 2 3 (Ser472 Ser473 Ser474)
- 浓度:
1mg ml
- 应用范围:
WB, IHC-P
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat
- 标记物:
HRP
- 克隆性:
Polyclonal
- 保存条件:
Store at 4°C for 12 months.
- 形态:
liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic phosphopeptide aa 450-480 480 derived from human AKT1 around the phosphorylation site of Ser473
- 规格:
100ul
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文献和实验v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
3.1 and 3.6 ). This will provide a “specific activity” measurement relative to total protein in the cell, and is themost accurate measure of total GSK3 activity, or2. Immunoblot using antibodies to phospho-Ser21 (GSK3a)or phospho-Ser9 (GSK3b) (see Sections 3.1 –
can then be treated with a variety of secondary antibodies to visualise the anti-BrdU labelled cells (Fig 1 and Fig 2) including HRP conjugated secondaries with diaminobenzidine(DAB) or fluorescent conjugated antibodies such as AlexaFlour 488 or 533. Tissue
Synthesis and Probing of Membrane-bound Peptide Arrays
with MBS for 2 hr at room temperature. Incubate the nitrocellulose membrane for 75 min with an AP- or HRP-conjugated detection antibody, or AP-/HRP-streptavidin for biotinylated proteins diluted in MBS. Use dilutions
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