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从原料到标记,打造属于中国自己的流式抗体品牌Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
AKT1 2 3 (Phospho-Ser472 Ser473 Ser474) Antibody HRP Conjugated
- 靶点:
AKT1 2 3 (Ser472 Ser473 Ser474)
- 浓度:
1mg ml
- 应用范围:
WB, IHC-P
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat
- 标记物:
HRP
- 克隆性:
Polyclonal
- 保存条件:
Store at 4°C for 12 months.
- 形态:
liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic phosphopeptide aa 450-480 480 derived from human AKT1 around the phosphorylation site of Ser473
- 规格:
100ul
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文献和实验v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
3.1 and 3.6 ). This will provide a “specific activity” measurement relative to total protein in the cell, and is themost accurate measure of total GSK3 activity, or2. Immunoblot using antibodies to phospho-Ser21 (GSK3a)or phospho-Ser9 (GSK3b) (see Sections 3.1 –
实验助攻 | WB 实验 (Western Blot) 杂带太多?原因就这几个!
产品推荐 Beta Actin 抗体 (HRP)(YA4634) www.medchemexpress.cn/antibody/beta-actin-antibody-hrp-conjugated.html 常用的内参抗体之一,可检测 Beta Actin 总蛋白的内源水平。 alpha Tubulin 抗体 (YA625) www.medchemexpress.cn/antibody/alpha-tubulin-rabbit-mab.html 可检测 alpha-tubulin 总蛋白的内源水平
can then be treated with a variety of secondary antibodies to visualise the anti-BrdU labelled cells (Fig 1 and Fig 2) including HRP conjugated secondaries with diaminobenzidine(DAB) or fluorescent conjugated antibodies such as AlexaFlour 488 or 533. Tissue
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