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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
干冰运输,-80℃保存
- 保质期:
6个月
- 英文名:
OmniMAX2-T1 Phage Resistant Competent Cel
- 库存:
60
- 供应商:
泽叶生物
- 规格:
0.1 mL×10
本产品是采用大肠杆菌OmniMAX2-T1 Phage Resistant菌株经特殊工艺处理得 到的感受态细胞,可用于DNA 的化学转 化。使用pUC19质粒检测,转化效率可达 109 。抗性为四环素抗性。
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文献和实验Standard Operating Procedures for T1-Phage Testing Assay
lawn, the lawn there will be lysed on incubation overnight. II. Materials and Equipments: DH10B (original glycerol stock) or DH10B cultured stock for phage test. DH10B-T1 phage infected cells (positive control).
Clone Genes From a Phage Library
The overall sequence of events is: • Titer and plate out phage • Lift
cDNA AMPLIFICATION FROM LAMBDA-PHAGE LIBRARY
1. Plate cells and phage as described (144. cDNA library screening) and let plaques develop 2. Pippet enough SM buffer on plates to barely cover the plate and rock at 4o C for >1 hour 3. Pippet solution containing phage
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