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- 保存条件:
低温运输,-20℃保存
- 保质期:
二年
- 英文名:
M13mp18 DNA
- 库存:
60
- 供应商:
泽叶生物
- 规格:
10 μg
该DNA为噬菌体 M13 衍生噬菌体载体, 共价闭环 DNA。A型为RF I型 双链DNA, 分子量7249bp,浓度为100μg/mL;B型 为单链DNA,大小为7249bases,浓度为 250μg/mL。具有β-半乳糖苷酶 13 个独 特的 RE 位点,可用于蓝/白斑筛选。
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文献和实验Transfection of E. coli with M13 DNA
In recent years several techniques have been described for the introduction of DNA molecules into strains of E. coli by transformation or transfection. These are based on the findings of Mandel and Higa (1 ) who demonstrated that incubation
Reverse Sequencing of M13 Cloned DNA
When shotgun sequencing projects are almost complete, continued random sequencing becomes increasingly less fruitful. The sequence may have regions of data that have only been determined on one strand (and therefore potentially error prone
Cloning DNA Fragments in M13 Vectors
The genome of the filamentous bacteriophage M13 comprises a single-stranded circular DNA molecule, designated the (+) strand, which is converted into a double-stranded replicative form (RF) on infection of Escherichia coli . Cloning vectors based
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