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- 技术资料
- 保存条件:
低温运输,-20℃保存
- 保质期:
一年
- 英文名:
Aminoally-dUTP Solution
- 库存:
60
- 供应商:
泽叶生物
- 规格:
100 μL
本产品是浓度为10 mM的 5-(3-氨基烯丙 基)-2’-脱氧鸟苷-5’-三磷酸的溶液,pH为 7.0。可在各种DNA聚合酶作用下掺入到新 合成的DNA产物中。
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文献和实验Fluorescent Sample Labeling for DNA Microarray Analyses
complementary DNA synthesis by reverse transcription. Label is introduced either by direct incorporation of fluorescently labeled nucleotides or indirectly by incorporation of aminoally-dUTP and subsequent coupling of fluorescent dyes. The third method is based
ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
Technologies; Cat # 48190-011) Cyanine 3-dUTP # NEL578 or Cyanine 5-dUTP #NEL579 (Perkin Elmer/NEN) B. REAGENT PREPARATION 50X dNTPs 50X [ ] Stock [ ] For 1.0 ml of 50X use:
PROTOCOL: •Deparaffinize and rehydrate slides: 3 x 3´ Xylene 3 x 2´ 100% ethanol 1 x 2´ 95%, 80%, 70% ethanol (each) 1 x 5´ 1x PBS •Microwave antigen retrieval: 4 x 5´ in microwave (600 ml of 10mM NaCitrate, pH 6) Cool 20
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